Potential activity of recombinant laccase for biodegradation of ampicillin

Abstract The broad applications of antibiotics in the field medicine and animal husbandry to treat bacterial infections humans animals have affected rise risks related antibiotic contamination. Since presence environment is harmful ecological system, this concern needs be addressed. Among various methods, biodegradation by harnessing specific enzymes such as laccase eliminate has attracted huge attention due its excellent ability performance. In study, laccase-encoding gene from Trametes hirsuta was introduced integrated into an expression host, Pichia pastoris . Furthermore, recombinant then investigated for potential activity degrade ampicillin. enzyme determined using syringaldazine a substrate, while ampicillin tested against Escherichia coli Staphylococcus aureus disk diffusion assay. could successfully expressed P. with highest at 716 U L −1 showed functionally deactivate antibacterial. This study indicates that use considered promising approach since it safe, sustainable, eco-friendly.